Cell Proliferation Reagent Wst 1

Measurement of cell proliferation in response to growth factors cytokines mitogens and nutrients. After incubation of pc12 cells with various concentrations of riv 0 1 1000 μm for an additional 24 h cell viability was assessed using the cell proliferation reagent wst 1 roche applied science mannheim germany according to the manufacturer s instructions. Cell biolabs cytoselect wst 1 cell proliferation assay reagent provides a colorimetric format for measuring and monitoring cell proliferation.

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Wst 1 Cell Proliferation Assay

The cell proliferation reagent wst 1 is used for the nonradioactive spectrophotometric quantification of cell proliferation and viability in cell populations using the 96 well plate format.

Cell proliferation reagent wst 1.

The larger the number of viable cells the higher. Roche s wst 1 cell proliferation reagent is a simple colorimetric assay designed to measure the relative proliferation rates of cells in culture. The assay principle is based on the conversion of the tetrazolium salt wst 1 into a colored dye by mitochondrial dehydrogenase enzymes. The wst 1 assay protocol is based on the cleavage of the tetrazolium salt wst 1 to formazan by cellular mitochondrial dehydrogenases.

It is also available in a ready to use reagent format. The rate of wst 1 cleavage by mitochondrial dehydrogenases correlates with the number of viable cells in the culture. Cells can be plated and then treated with compounds or agents that affect proliferation. Number of reactions cat.

93 100 based on 12 pubmed citations. Zero bias scores article reviews protocol conditions and more. 11 644 807 001. Roche cell proliferation reagent wst 1 cell proliferation reagent wst 1 supplied by roche used in various techniques.

Wst 1 assay reagent ab155902 provides a simple accurate and ready to use assay to measure cell proliferation cell viability and cytotoxicity in mammalian cells. Roche s cell proliferation reagent wst 1 is a water soluble tetrazolium salt that can be used for cell proliferation or cell viability assays. The cell proliferation reagent wst 1 is a clear slightly red ready to use solution containing wst 1 and an electron coupling reagent diluted in phosphate buffered saline filtered through 0 2 µm pore size membrane. Wst 1 cell proliferation assay kit ab65473 provides by far the easiest and most sensitive means for performing a quantitative cell proliferation assay cell viability assay or cytotoxicity assay in mammalian cells.

The 10 ml volume is sufficient for the evaluation of 960 assays in ten 96 well plates or 192 assays in eight 24 well plates. Wst 1 is added directly to the cells 1 10th of the culture volume and absorbance. The soluble salt is released into the media.